Specialized
council 042
Armenian society of biological psychiatry
Armenian association for molecular
and cellular biology and immunology
Eng Rus Arm

LABORATORY OF MOLECULAR GENETICS


HEAD:

Anahit Sedrakyan, PhD

E-mail: a_sedrakyan@mb.sci.am

Phone1: +374 93 459-954

Phone2: +374 10 231-499

Group members:

  1. Zhanna Ktsoyan, PhD
  2. Alvard Hovhannisyan, PhD
  3. Mkhitar Mkrtchyan, PhD
  4. Karine Arakelova
  5. Magdalina Zakharyan

General information:

The research interests of the laboratory are focused on the study of host-microbiota interactions in normal and various pathological conditions.

Over the past two decades, due to the cooperation with the "Nork" clinical hospital of infectious diseases (MH, Armenia), the monitoring of antimicrobial resistance in clinical non-typhoidal Salmonella enterica isolates and the study of the immune status of patients with salmonellosis were carried out. In particular, we discovered that the profile of inflammatory responses during the Salmonella infection depend on the serotype of the pathogen. Our results suggest that nontyphoidal Salmonella infection contributes to the generation of autoantibodies and may play a role in autoimmune disease.

Within the frame of the ISTC Project A-2140 (2016-2019), WGS and annotation of 44 clinical non-typhoidal Salmonella enterica isolates from Armenia was carried out for the first time. Extensive data were obtained that characterize the genetic patterns resposible for virulence and antimicrobial resistance of these isolates.

Current research activities of the laboratory are focused on research project studying the most common foodborne bacterial pathogens circulating in Armenia for developing the appropriate strategies to control the most common foodborne diseases in Armenia. One of the research tasks of the laboratory is to study the potential of bacteriophages to control bacterial infections and spread of antimicrobial resistance.

Current research projects:

1.

#18T-1F405 Project "Development of approaches for bacteriophage based prophylaxis and treatment of the prevalent in Armenia foodborne infectious diseases of bacterial origin" 2018-2020 (Committee of Science, Ministry of Education, Science, Culture and Sport RA), PI: Anahit Sedrakyan


The main goals of the project are: generation of genetic blueprints of the most common foodborne bacterial pathogens in Armenia and development of bacteriophage based preparations active against these pathogens for efficient prophylaxis and therapy of the diseases.


Foodborne diseases are a major public health concern and an important cause of morbidity in Armenia, especially for children under 5 years old. Because of the growing problem of antibiotic resistance, other approaches beyond the traditional boundaries of antibiotic therapy are needed to control future outbreaks. Nowadays, one of the most promising approaches for prophylaxis and treatment of foodborne diseases is phage therapy, which has some unique advantages over antibiotics. This project will target the foodborne pathogens frequently isolated from patients in Armenia including Shigella, non-typhoidal Salmonella, Klebsiella, Staphylococcus aureus, Pseudomonas aeruginosa, and pathogenic Escherichia coli. Studies will focus on the molecular aspects of antimicrobial resistance to identify and characterize emerging resistance phenotypes and genotypes of these high priority type bacteria. Within the frame of the project, the antimicrobials that are active against the foodborne pathogens frequently isolated from patients in Armenia were identified. Based on the results obtained, we selected the highly virulent and multidrug resistant (MDR) clinical isolates for more detailed investigation including whole genome sequencing (WGS) to characterize antimicrobial resistance and track specific clones of pathogens. WGS and annotation of foodborne pathogens (except for Salmonella) will be performed for the first time in Armenia. Bacteriophages with lytic activity against the foodborne bacterial pathogens will be isolated and WGS of the selected phages will be performed to address potential risks related to phage application. The collection of phage clones isolated in Armenia generated within the frame of the project will be used for the development of phage-based preparations for prophylaxis and treatment of foodborne diseases.


2.

ANSEF (Armenian National Science & Education Fund) Project "Next step: Addressing the risks of phage therapy", 2019-2020, PI: Mkhitar Mkrtchyan

Selected Publications:

>> Full publications list of IMB
  1. Zhanna Ktsoyan, Lyudmila Budaghyan, Marina Agababova, Armine Mnatsakanyan, Karine Arakelova, Zaruhi Gevorgyan, Anahit Sedrakyan, Alvard Hovhannisyan, Mkhitar Mkrtchyan, Magdalina Zakharyan, Arsen Arakelyan and Rustam Aminov "Potential Involvement of Salmonella Infection in Autoimmunity" Pathogens 2019, Volume 8, Issue 3, 96; doi:10.3390/pathogens8030096
  2. Kakabadze E., Makalatia K., Grdzelishvili N., Bakuradze N., Goderdzishvili M., Kusradze I., Phoba M.-F., Lunguya O., Lood C., Lavigne R., Jacobs J., Deborggraeve S., De Block T., Van Puyvelde S., Lee, D., Coffey A., Sedrakyan A., Soentjens P., De Vos D., Pirnay J.-P., Chanishvili N, Selection of Potential Therapeutic Bacteriophages that Lyse a CTX-M-15 Extended Spectrum β-Lactamase Producing Salmonella enterica Serovar Typhi Strain from the Democratic Republic of the Congo, "Viruses", v.10, N4, 2018, p.172; doi:10.3390/v10040172
  3. Ktsoyan Zh., Mkrtchyan M., Zakharyan M., Mnatsakanyan A., Arakelova K., Gevorgyan Z., Sedrakyan A., Hovhannisyan A., Arakelyan A., Aminov R., Systemic concentrations of short chain fatty acids are elevated in salmonellosis and exacerbation of familial Mediterranean fever. // Frontiers in Microbiology, 2016, vol. 7; DOI:10.3389/fmicb.2016.00776
  4. Ktsoyan Zh., Mkrtchyan M., Zakharyan M., Mnatsakanyan A., Arakelova K., Gevorgyan Z., Ktsoyan L., Sedrakyan A., Hovhannisyan A., Ghazaryan K., Boyajyan A., Aminov R., Differential induction of total IgE by two Salmonella enterica serotypes. // Frontiers in Cellular and Infection Microbiology, 2015, vol. 5; doi:10.3389/fcimb.2015.00043
  5. Ktsoyan Zh., Beloborodova N., Sedrakyan A., Osipov G., Khachatryan Z., Manukyan G., Arakelova K., Hovhannisyan A., Arakelyan A., Ghazaryan K., Zakaryan M., Aminov R., Management of familial Mediterranean fever by colchicine does not normalize the altered profile of microbial long chain fatty acids in the human metabolome. // Frontiers in Cellular and Infection Microbiology, 2013, vol. 3; doi:10.3389/fcimb.2013.00002
  6. Ktsoyan, Zh., Ghazaryan, K., Manukyan, G., Martirosyan, A., Mnatsakanyan, A., Arakelova, K., Aminov, R., Inflammatory Responses to Salmonella Infections Are Serotype-Specific. // International Journal of Bacteriology, 2013. doi.org/10.1155/2013/168179