Comparison of in vitro antiviral activity of flavones against African swine fever virus and structure-activity analysis.
Recently we found that apigenin chemically known as 4′, 5, 7,-trihydroxyflavone has significant anti-ASFV
activity, reducing the viral yield of approximately 1000-fold during in vitro infection (Hakobyan et al., submitted). Apigenin is a non-toxic dietary
flavone abundantly found in parsley, basil, celery, chamomile and other plants. Natural flavones also include luteolin, tangeritin, chrysin, 6-hydroxyflavone,
baicalein, scutellarein and some other compounds. Thus, further screening of these compounds may reveal new antivirals and structural motifs associated with
Screening of rigid amphipatic fusion inhibitors (RAFIs) against African swine fever virus.
Our collaborators from Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russia) described a family
of compounds that inhibit virus infectivity through targeting virion envelope lipids and preventing the fusion of cellular and viral membranes. We are screening
15 different RAFIs in order to identify effective antivirals targeting ASFV entry to the host cell. This project is supported by research grant (15RF-081)
from State Committee of Science.
Development of colorimetric assay for high-throughput screening of inhibitors against African swine fever virus.
Currently available in vitro assay systems evaluating compounds associated with anti-ASFV activity are tedious
and time consuming to perform. Our group will develop a quantitative colorimetric assay adapted to a microtiter plate format for determination of ASFV
susceptibility to antiviral compounds. This assay will allow us to rapidly perform large-scale screening programs for anti-ASFV agents using a microtiter